FastQCFastQC Report
Thu 4 Nov 2021
H5GH5BGXK_n01_CD41_Low_R2__CD41_Input_R2__CD41_Veh_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GH5BGXK_n01_CD41_Low_R2__CD41_Input_R2__CD41_Veh_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31203094
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTACCAA27744628.891624657477877No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTTCCAA20658516.620660758833723No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTACCAAC19771616.3364261249221No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTAGCAAC11177203.5820806744356823No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTACCAAC10252703.2857959534397456No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACT9750223.124760640723641No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTAGCAAC7573542.4271759717161383No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGAACCCCTACCAACT6226631.9955168548349727No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAATCACCGAACCCCTACCAAC6059661.942006135673597No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGTACCCCTACCAA5975631.9150761139263945No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGATACACCGAACCCCTACCAA5882891.8853547023253525No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGTTACACCGAACCCCTTCCAA5696331.8255657596006343No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGAACCCCTACGAACT5337561.7105867770676841No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGATCCCCTAGCAAC4858541.5570699495376965No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGAACCCCTACGAACT4719131.512391687824291No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGTACCCCTTCCAA4707111.5085395057297843No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGATCCCCTAGCAAC4509441.4451900186564832No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGAACCCCTACCAAC4388131.4063124637576003No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGAACCCCTACGAACT4362281.3980280288871354No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGATACACCGAACCCCTTCCAA4303771.3792766832673709No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGTACCCCTACCAAC4031661.2920705876154461No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACT3914671.2545775108071013No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGATCCCCTACCAAC3883961.244735538084781No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGAACCCCTACGAACT3808031.2204014127573375No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGAACCCCTACCAACT3617471.1593305458747136No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAAACACCGAACCCCTACCAAC3276901.050184318260234No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA3124441.0013237789816614No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACGAACT3055040.9790823948419988No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT3003500.9625648020673847No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGATCCCCTACCAAC2659590.8523481677810539No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGAACCCCTAGCAAC2533290.811871412495184No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGAATCACCGAACCCCTACCAAC2265800.7261459392456402No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGAACCCCTACCAACT2179540.6985012447804054No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGTACCCCTAGCAAC2168680.6950208206916917No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGTTACACCGTACCCCTTCCAA2099960.6729973636588731No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGAACCCCTACGAACT2083450.6677062216971177No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGTACCCCTACCAAC2061040.6605242416024514No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGTACCCCTACCAACT2021650.6479004934574757No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGTTACACCGAACCCCTTCCAA1543170.49455672568880515No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATGACCGAACCCCTACGAACT1468850.4707385748349186No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGTACCCCTAGCAAC1459040.4675946558376551No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACGAACT1449620.4645757244457874No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGAACCCCTACCAACT1381420.44271891755349646No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAATCACCGTACCCCTACCAAC1376700.4412062470471679No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATACACCGAACCCCTACCAAC1367820.4383603754166174No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACGAACT1327860.4255539530791401No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACGAACT1325620.42483607555071307No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGTTACACCGAACCCCTACCAA1321880.4236374764630712No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGATTCACCGAACCCCTACCAAC1290260.4135038659948273No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGATACACCGTACCCCTACCAA1287460.41260651908429335No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACCAACT1269010.4066936439059537No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAATCACCGAACCCCTAGCAAC1163230.3727931595501395No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACGAACT1157840.3710657667473617No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGAACCCCTACCAAC1100540.3527022031853636No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGATTCACCGAACCCCTAGCAAC1068430.3424115570077762No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATGACCGAACCCCTACGAACT1045060.33492191511521263No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGATACACCGTACCCCTTCCAA1021240.3272880567548846No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGATCCCCTAGCAAC986070.3160167385964995No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACGAACT975080.31249465197265375No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGTTCCCCTAGCAAC964390.309068709660651No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGAACCCCTACCAACT956030.30638948817062817No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATCACCGAACCCCTACGAACT931600.29856013637621964No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGTACCCCTACCAAC929170.29778136745029193No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGTTCCCCTAGCAAC880880.28230533805397634No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGTTCCCCTACCAAC830890.2662844908905508No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGAAACACCGAACCCCTACCAAC798000.2557438695021718No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGTACCCCTACCAACT784670.2514718572459513No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT763360.24464240629470912No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATCACCGAACCCCTACCAACT762890.24449178020615522No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGATCCCCTACCAAC744210.23850519438873594No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACCAACT724770.23227504298131463No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAA705210.2260064338491561No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACGAACT676100.21667723078999793No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAAACACCGTACCCCTACCAAC641280.20551808099542948No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGTTACACCGTACCCCTTCCAA629420.2017171758672393No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGATTCACCGATCCCCTAGCAAC629320.20168512776329167No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGAAACACCGAACCCCTACCAA622030.19934882098550868No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT615850.1973682481615445No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGTACCCCTACGAACT614690.19699649015575185No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGTTCCCCTACCAAC561070.17981229681902697No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC559890.17943412919244484No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGTACCCCTAGCAAC514510.16489069962100553No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACCAACT506200.1622275021829566No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAAGACCGAACCCCTACCAACT502880.16116350513189492No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATACACCGAACCCCTACCAAC500200.16030461594609816No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGAATCACCGTACCCCTACCAAC491290.1574491298843634No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATGACCGTACCCCTACGAACT462200.14812633644599474No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAAGACCGAACCCCTACGAACT455160.14587014992808084No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGAATCACCGAACCCCTAGCAAC454410.14562978914847355No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGTACCCCTACCAACT445340.14272302612042254No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATGACCGAACCCCTACCAACT410010.13140043099572113No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAATCACCGATCCCCTACCAAC399320.12797448868371836No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGTACCCCTACGAACT390030.12499721982698254No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACGAACT354030.11345990240583194No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGATTCACCGATCCCCTACCAAC350220.11223886964542684No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGTTACACCGAACCCCTACCAA347640.11141202856357771No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTACCAA341530.10945388941237687No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACCAACT338110.10835784425736755No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGTTACACCGTACCCCTACCAA331110.10611447698103273No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)