FastQCFastQC Report
Thu 4 Nov 2021
H5GH5BGXK_n01_CD41_Input_R1__CD41_Veh_R1__CD41_High_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GH5BGXK_n01_CD41_Input_R1__CD41_Veh_R1__CD41_High_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25074825
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTACCAA23674829.4416690844303No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTACCAAC15116666.0286203393244016No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTTCCAA14653365.84385334693263No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACT7970843.1788217863933244No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAATCACCGAACCCCTACCAAC7165502.8576470623424095No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTACCAAC6600452.6323015215460126No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTAGCAAC6079882.4246948881996184No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAAACACCGAACCCCTACCAAC5998252.3921403240102372No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGAACCCCTACCAACT5485372.1876005116685757No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGTACCCCTACCAA5061192.0184348245700616No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGAACCCCTACGAACT4862271.9391042609469857No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGATACACCGAACCCCTACCAA4735871.8886951354595696No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGAACCCCTACGAACT4652001.8552472449957276No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGAACCCCTACCAAC4242731.6920277609115915No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGAACCCCTACGAACT4134811.648988577188475No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGAACCCCTACGAACT3630311.44779076224859No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACT3469181.3835310914433103No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGTTACACCGAACCCCTTCCAA3463561.3812897996297082No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA3462981.3810584919336426No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGAACCCCTAGCAAC3424411.3656765301452751No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGAACCCCTACCAACT3341601.3326513744363122No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGATACACCGTACCCCTTCCAA3319981.324029180662278No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGTACCCCTACCAAC3259021.2997179441930302No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGATACACCGAACCCCTTCCAA2913431.1618944499114152No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACGAACT2645901.0552017810692598No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGATCCCCTACCAAC2471500.9856499497005462No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGATCCCCTAGCAAC2441090.9735222479119994No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT2265850.9036354191903632No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGAATCACCGAACCCCTACCAAC2220380.8855016934315594No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGATCCCCTAGCAAC1923030.766916618560648No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGAACCCCTACCAAC1761380.7024495684416541No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGAACCCCTACCAACT1736740.6926229794225882No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGAACCCCTAGCAAC1729160.6896000271188333No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGAACCCCTACGAACT1715240.6840486424132571No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAATCACCGTACCCCTACCAAC1706960.6807465256487334No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATACACCGAACCCCTACCAAC1582550.6311310248426459No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGTACCCCTACCAACT1546870.6169016134708817No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGTACCCCTACCAAC1445240.57637092183096No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGATCCCCTACCAAC1444490.5760718170515646No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC1372020.5471703192345311No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACGAACT1335890.5327614449951296No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACGAACT1309750.5223366464172731No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGTTACACCGTACCCCTTCCAA1271900.5072418252171251No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGAACCCCTACCAACT1268250.5057861819574014No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAAACACCGTACCCCTACCAAC1262060.5033175705114592No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATGACCGAACCCCTACGAACT1262040.5033095943840087No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACGAACT1259780.5024082919820976No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGTACCCCTAGCAAC1258320.5018260346782082No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGAAACACCGAACCCCTACCAAC1123400.4480190788968617No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACCAACT1073620.4281664976724663No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGATACACCGTACCCCTACCAA1026410.40933884882546534No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGATTCACCGAACCCCTACCAAC1016310.40531090446294243No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGTACCCCTACCAAC935900.37324288404804423No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATGACCGAACCCCTACGAACT933960.37246919968534176No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGTTACACCGAACCCCTTCCAA919780.3668141253229085No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGAACCCCTACCAACT915530.3651191982396687No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACGAACT908540.36233154169570475No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACGAACT865800.3452865573338996No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAATCACCGAACCCCTAGCAAC857460.3419605121870242No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGTTACACCGAACCCCTACCAA825610.32925852922203847No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATCACCGAACCCCTACGAACT805230.3211308553499376No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAA777260.3099762411103567No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGTACCCCTAGCAAC735880.29347363341518834No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGATACACCGTACCCCTTCCAA689030.27478955486229717No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGTACCCCTACCAACT652540.26023711032878594No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATCACCGAACCCCTACCAACT647510.25823111427497497No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACCAACT643210.2565162468731088No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGAAACACCGAACCCCTACCAA639270.2549449497653523No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGTACCCCTACGAACT631740.2519419377802238No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT619660.2471243568000973No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGATCCCCTAGCAAC614650.24512633687373692No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGATCCCCTACCAAC601580.23991393758480867No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGATTCACCGAACCCCTAGCAAC597700.23836656885940383No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGTTCCCCTACCAAC585300.23342136984006867No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATTCACCGTTCCCCTAGCAAC527850.210509943738391No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGAATCACCGTACCCCTACCAAC524820.2093015604296341No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGTACCCCTACGAACT506820.20212304572414763No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATACACCGAACCCCTACCAAC492170.19628053236662668No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACCAACT453630.18091053476943508No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATACACCGAACCCCTACCAAC441780.17618467925498982No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT433880.1730341089120263No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAATCACCGTACCCCTACCAAC423970.16908193776028346No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAAGACCGAACCCCTACGAACT420850.16783766187799914No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAAGACCGAACCCCTACCAACT419430.16727135682901076No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGTTCCCCTAGCAAC415010.1655086326624413No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATGACCGTACCCCTACGAACT396730.15821845217264727No Hit
ATCGATACCTTGGTTCATGTGGAAAGGACGTTACACCGTACCCCTTCCAA370690.14783353423204348No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGATTCACCGTACCCCTAGCAAC367780.14667300768798985No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGTACCCCTACCAACT344060.13721332053164878No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATGACCGAACCCCTACCAACT343370.13693814413460514No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGTTCCCCTACCAAC343290.13690623962480297No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGATACACCGAACCCCTACCAA333840.13313751940442256No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGATTCACCGATCCCCTAGCAAC326210.13009462678204134No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAATCACCGATCCCCTACCAAC324440.1293887395026685No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAATCACCGTACCCCTACGAACT313180.12489817974801419No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATGACCGTACCCCTACCAACT311820.12435580308137743No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAATCACCGAACCCCTACCAAC305910.1219988574197427No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGATACACCGTACCCCTACCAAC296710.11832983879249406No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACGAACT293190.11692604036119894No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAC288450.11503569815542083No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATGACCGTACCCCTACGAACT270760.10798081342541772No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAAGACCGAACCCCTACGAACT265290.1057993425676949No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGAATCACCGAACCCCTAGCAAC263310.10500970595009138No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAAGACCGAACCCCTACCAACT252120.10054706264151395No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)