FastQCFastQC Report
Thu 4 Nov 2021
H5GH5BGXK_n01_CD235_Veh_R2__CD235_Input_R1__CD235_Input_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GH5BGXK_n01_CD235_Veh_R2__CD235_Input_R1__CD235_Input_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10418136
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT164325515.773023120450722No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCTACTG7669747.361911958146831No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG6599156.334290510317777No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTG5777715.545819328908742No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCAACTG5129544.923663887666661No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCATCTGG4793254.600871019537468No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT3437013.299064247193548No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACCGAACCCCTACCAACTGGT3123142.997791543516038No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCTACTG2709562.600810740040253No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGAACCCCTACCATCTGG2631552.5259317021778176No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCTACTG2194382.106307692662104No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG1852501.7781491813890702No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT1780221.7087701677152227No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCTACTG1665001.5981745678881518No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTG1600581.5363400900122632No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG1591781.5278932814852868No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCATCTGG1433501.375965911752352No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCAACTGG1300601.2483999057029012No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGTACCCCTACCAACTG1148481.1023853019388499No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCAACTG1145741.099755272920223No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAAGTCCGAACCCCTACCTACTG1037500.9958595280384129No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCATCTGG943210.9053538944010713No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGAACCCCTACCAACTGG941220.9034437638364482No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCATCTGG846860.8128709396767331No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCTACTG814240.7815601562505998No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCTACTG777870.7466498805544486No Hit
TAAGTACAGTCTTGTGCAAAGGACGAAACACCGAACCCCTACCAACTGGT647780.6217810940459982No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT646280.6203412971379909No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGAACCCCTACCATCTGG609020.5845767419430885No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCTACTG596610.5726648221908411No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAAGTCCGAACCCCTACCTACTG586580.5630373801992986No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCATCTGG558870.5364395319853763No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCATCTGG433270.4158805375548947No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGTACCCCTACCTACTG426350.40923827448595407No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTCCGAACCCCTACCATCTGG379430.36420142720348436No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG365020.35036977824056054No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG363350.3487668043496457No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT356390.34208614669649157No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTG355510.34124146584379395No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAAGTCCGTACCCCTACCTACTG327450.3143076650180032No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAGTGGT324080.3110729212980134No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAAGTCCGAACCCCTACCTACTG316700.3039891205106172No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG313030.3004664174090259No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTGG307470.2951295702033454No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCAACTG289440.27782321136909716No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCATCTGG283330.2719584386304806No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACAGAACCCCTACCAACTGGT282810.27145930903570464No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCATCTGG274610.26358841927193116No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGCGAACCCCTACCATCTGG260770.2503038931340501No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTGCGAACCCCTACCATCTGG235260.22581774705187188No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCAACTGG221240.21236044528502987No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAACTGGT200410.19236646555583456No Hit
GATCGCTCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCTACTG187890.1803489606969999No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTG185750.1782948504415761No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCATCTGG181690.1743978001439029No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCTACTG172640.1657110254655919No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGCGAACCCCTACCATCTGG172000.16509671211817548No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCTACTG167760.16102688619154135No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAAGTCCGAACGCCTACCTACTG152010.14590901865746425No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCTACTG150030.14400848673889455No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGCGAACCCCTACCAACTGG148120.14217514534269854No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAAGTCCGTACCCCTACCTACTG147980.1420407642979512No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAACTGGT136020.13056078361810597No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAGTGGT134750.12934175556932642No Hit
TAAGTACAGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT130310.12507995672162467No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAAGTCCGAACGCCTACCTACTG125030.12001187160543883No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAAGTCCGAACCCCTACCTACTG118440.1136863638562599No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAAGTGGT116570.1118914170442774No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACTGGT115270.1106435930573377No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGCGTACCCCTACCATCTGG109570.10517236480690981No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAAGTGGT107470.10315664913569951No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAAGTCCGTACCCCTACCTACTG105210.10098735512763511No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)