FastQCFastQC Report
Thu 4 Nov 2021
H5GH5BGXK_n01_CD11b_Veh_R1__THP1_PMA_R1__CD11b_High_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GH5BGXK_n01_CD11b_Veh_R1__THP1_PMA_R1__CD11b_High_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16069755
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT164092710.21127577862886No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCTACTG15161499.43479847701474No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG9314405.7962302474431No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCATCTGG8550315.320746956005241No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCAACTG7979944.965813106671508No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTG6576524.092483052790786No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGAACCCCTACCATCTGG5844473.6369378375712635No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCTACTG4949463.079984729076455No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT4005982.492869368574692No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACCGAACCCCTACCAACTGGT3462262.1545194684050877No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCTACTG3039241.8912796119169208No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCTACTG3020801.8798046392119856No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAAGTCCGAACCCCTACCTACTG2645651.6463536625169455No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT2558271.5919782224433416No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG2440601.518753708441728No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCATCTGG2364311.4712794314536843No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTG2290551.4253795406339425No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCTACTG1979441.2317798248946545No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGAACCCCTACCAACTGG1883581.1721273908656353No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCAACTGG1812951.128175258428022No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCATCTGG1784331.1103654038284965No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCAACTG1674701.0421440774921584No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG1637761.0191567948609048No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCATCTGG1626621.0122245174241922No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAAGTCCGAACCCCTACCTACTG1477840.919640654135673No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGAACCCCTACCATCTGG1473360.9168528082724346No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGTACCCCTACCAACTG1262540.7856622580742519No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCATCTGG1182310.735736170215414No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCTACTG995370.6194058341275271No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCTACTG964820.6003949655735262No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTCCGAACCCCTACCATCTGG956440.5951802003204155No Hit
TAAGTACAGTCTTGTGCAAAGGACGAAACACCGAACCCCTACCAACTGGT953650.5934440195261222No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCATCTGG952490.5927221665793909No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT836780.520717335142944No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAAGTCCGAACCCCTACCTACTG788440.4906359804489864No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAAGTCCGTACCCCTACCTACTG774810.48215420832489353No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG676240.4208153764634246No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCATCTGG646480.4022961146576286No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTGCGAACCCCTACCATCTGG589710.3669688803594081No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT587240.36543183141248886No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTGG584640.3638138851525739No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCAACTG563620.35073341192818436No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGTACCCCTACCTACTG559570.34821314948485527No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGCGAACCCCTACCATCTGG529880.32973744777067227No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAGTGGT514290.3200359930814129No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTG478580.2978141234885037No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG475750.29605305121328856No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCATCTGG456900.28432294082890497No Hit
GATCGCTCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCTACTG428960.2669362414050494No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCAACTGG426410.26534940949628666No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCTACTG400020.24892725495814966No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGCGAACCCCTACCATCTGG397830.2475644463776828No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAAGTCCGAACGCCTACCTACTG367330.22858469217483401No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCATCTGG366700.22819265135031616No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCTACTG366480.22805574820524643No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAAGTCCGTACCCCTACCTACTG359660.22381175070808487No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG312580.19451447766316288No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAACTGGT307690.19147149412047662No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAAGTCCGAACGCCTACCTACTG303610.1889325630664562No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACAGAACCCCTACCAACTGGT294070.18299594486661433No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAAGTCCGAACCCCTACCTACTG293010.18233632062218746No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTG281160.17496221939911344No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAAGTCCGTACCCCTACCTACTG238230.14824743749982497No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGCGAACCCCTACCAACTGG233380.14522934543806051No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAGTGGT230380.14336248436892784No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGAACCGCTACCATCTGG228920.14245394531528327No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGCGTACCCCTACCATCTGG228010.14188766412431303No Hit
TAAGTACAGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT216240.13456334586308252No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTCCGAACCCCTACCAACTGG214900.1337294812522033No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGAACCGCTACCATCTGG214600.13354279514529002No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAACTGGT212780.13241023276334954No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACTGGT210690.1311096528851871No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCATCTGG198890.12376666601326529No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCATCTGG193980.12071123673011816No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCTACTG187710.1168094970956309No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGCGTACCCCTACCATCTGG183430.11414610863700163No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCTACTG178250.11092266185763257No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTGCGAACCGCTACCATCTGG177610.11052439816288426No Hit
GATCGCACGGTTCTTGAGGAAAGGACGAAAGTCCGAACCCCTACCTACTG174020.1082903877501555No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAAGTGGT172510.10735073434535872No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCATCTGG170620.10617461187180513No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCATCTGG170180.10590080558166567No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)