FastQCFastQC Report
Thu 4 Nov 2021
H5GH5BGXK_n01_CD11b_Low_R2__CD11b_Input_R2__CD11b_Veh_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GH5BGXK_n01_CD11b_Low_R2__CD11b_Input_R2__CD11b_Veh_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22503198
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT352484915.663769211824915No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG15272836.786959791226119No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCTACTG14967096.651094657745979No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCATCTGG12549605.576807349782017No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCAACTG9013454.005408475719762No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTG8753303.889802684933937No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGAACCCCTACCATCTGG7714453.4281571890359763No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT7243623.218929149536879No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACCGAACCCCTACCAACTGGT6603802.934605117014924No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCTACTG5417882.4076044658185918No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG4426341.9669826484217932No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT4111321.826993656634937No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCATCTGG3847351.7096903293478554No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCTACTG3633981.6148726949831753No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCTACTG3251191.4447679836439247No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCAACTGG3014501.3395873777584857No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTG2879851.2797514379956128No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGAACCCCTACCAACTGG2616761.1628391662376165No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCATCTGG2566161.140353473315215No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG2474411.0995814905952477No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCATCTGG2436381.0826816704008027No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAAGTCCGAACCCCTACCTACTG2233200.9923922813104163No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCAACTG2045060.9087863867171235No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGAACCCCTACCATCTGG1820370.8089383562283013No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGTACCCCTACCAACTG1801450.8005306623529687No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCATCTGG1602190.7119832478921441No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCTACTG1589990.7065617962389168No Hit
TAAGTACAGTCTTGTGCAAAGGACGAAACACCGAACCCCTACCAACTGGT1450330.6444995062479564No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT1350320.6000569341299846No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCTACTG1310200.5822283570539618No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCTACTG1190550.5290581365368603No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAAGTCCGAACCCCTACCTACTG1146630.5095409105852422No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCATCTGG1141580.5072967851058324No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTCCGAACCCCTACCATCTGG1069950.47546575380085976No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTGG886340.3938729064197897No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG875130.3888913922367834No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG874650.38867808922091873No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAGTGGT854790.37985267693951763No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCATCTGG814560.3619752179223593No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT804330.35742919739674334No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCATCTGG748370.33256162079718626No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGCGAACCCCTACCATCTGG722370.3210077074378495No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGTACCCCTACCTACTG721250.3205100004008319No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAAGTCCGTACCCCTACCTACTG717430.3188124638995755No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAAGTCCGAACCCCTACCTACTG715800.3180881224082017No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTGCGAACCCCTACCATCTGG656710.29182963239269366No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTG654620.2909008755111162No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACAGAACCCCTACCAACTGGT638700.28382632548493775No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCAACTGG597880.26568668151077907No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCATCTGG538880.23946818581074567No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGCGAACCCCTACCATCTGG527050.23421115523224742No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAACTGGT526530.2339800769650607No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCAACTG514940.2288296978944948No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG507400.22547906302028717No Hit
GATCGCTCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCTACTG386400.17170892777106614No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGCGAACCCCTACCAACTGG356960.15862634279803253No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCTACTG347690.15450692830414592No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAGTGGT333820.1483433599082228No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAACTGGT326660.1451615899215747No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCTACTG323360.14369513168750503No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGCGTACCCCTACCATCTGG306280.1361050993729869No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTG298760.13276335212444026No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAAGTCCGTACCCCTACCTACTG298300.13255893673423663No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAAGTCCGAACGCCTACCTACTG294360.13080807447901405No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACTGGT294240.1307547487250479No Hit
TAAGTACAGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT282770.12565769540844818No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGAACCGCTACCATCTGG280590.12468894421139608No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAAGTGGT273230.12141829796813769No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGAACCGCTACCATCTGG272870.12125832070623917No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAAGTGGT270790.12033400763749225No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCTACTG258530.11488589310728191No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAAGTCCGAACGCCTACCTACTG257070.11423709643402684No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTCCGAACCCCTACCAACTGG254580.11313058703922882No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGCGTACCCCTACCATCTGG248060.11023322107373361No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAAGTCCGAACCCCTACCTACTG243690.10829127486679893No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAAGTCCGTACCCCTACCTACTG234460.10418963562423439No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCATCTGG226850.10080789406021312No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)