FastQCFastQC Report
Thu 4 Nov 2021
H5GH5BGXK_n01_CD11b_High_R1__CD11b_Low_R1__CD11b_Input_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GH5BGXK_n01_CD11b_High_R1__CD11b_Low_R1__CD11b_Input_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23622348
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT457709119.37610520342855No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG17874077.566593295467496No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCATCTGG15907476.734076561737217No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGAACCCCTACCATCTGG10607024.4902479634962615No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT9386793.9736905069724653No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCTACTG9309903.9411408213950625No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACCGAACCCCTACCAACTGGT9074713.841578322358133No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT6319812.6753521707494956No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG4889632.0699170124832635No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCAACTG4745002.0086910920116834No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCATCTGG4636801.9628870085226076No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTG3811031.6133154926004816No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGAACCCCTACCAACTGG3487601.4763985358271752No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCATCTGG3392641.4361993143103302No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCAACTGG3096941.3110212414108877No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCTACTG3062161.296297895535194No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCATCTGG2710951.1476208884908479No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGAACCCCTACCATCTGG2609891.1048393665185188No Hit
TAAGTACAGTCTTGTGCAAAGGACGAAACACCGAACCCCTACCAACTGGT2281630.9658777357780014No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCATCTGG1946230.8238935435207373No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCTACTG1903430.8057751075379975No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT1852300.7841303497857199No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCTACTG1789550.7575665213297171No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAAGTCCGAACCCCTACCTACTG1683100.7125032617418048No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCATCTGG1616570.684339253659289No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTCCGAACCCCTACCATCTGG1598960.6768844485738674No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTG1371840.5807382060411607No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAGTGGT1331690.5637415891087542No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT1226500.5192117227296795No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCTACTG1213920.5138862572001733No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG1170200.4953783595093934No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTGG1132190.47928766437612386No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCATCTGG1049780.4444012085504794No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGTACCCCTACCAACTG1020820.4321416313060836No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTGCGAACCCCTACCATCTGG982580.4159535707458039No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGCGAACCCCTACCATCTGG969060.41023017694938707No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG958830.4058995320871574No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAAGTCCGAACCCCTACCTACTG926000.39200167570133165No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG849160.3594731565211045No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACAGAACCCCTACCAACTGGT841160.35608653297292886No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAACTGGT811770.34364492471281854No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCATCTGG793940.3360969874798221No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGTACCCCTACCAACTG744210.31504488884847515No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGCGAACCCCTACCATCTGG736810.3119122620664127No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCAACTGG701040.29676982152663234No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCATCTGG629850.2666331052273042No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCTACTG624510.264372534008897No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCTACTG575130.2434686001577828No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAGTGGT552100.23371935761847215No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAACTGGT521750.22087135453258078No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAAGTCCGTACCCCTACCTACTG519520.21992733321852678No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAAGTCCGAACCCCTACCTACTG518590.2195336382310514No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACTGGT461300.1952811803466785No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGCGAACCCCTACCAACTGG449980.19048910802600993No Hit
TAAGTACAGTCTTGTGCAAAGGACGAAACACCGTACCCCTACCAACTGGT437130.1850493439517528No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAAGTGGT426140.18039696985244652No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTGCGAACCGCTACCATCTGG409650.17341629206376943No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAAGTGGT409500.17335279287224115No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGAACCGCTACCATCTGG384610.16281616035797966No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACAGCGTACCCCTACCATCTGG372100.15752032778452No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTCCGAACCCCTACCAACTGG369970.15661863926481823No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCATCTGG336740.14255145170158362No Hit
GATCGCCCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCAACTG334990.1418106278004202No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGTACCCCTACCTACTG334330.14153123135769569No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTGCGTACCCCTACCATCTGG308390.13055010450273613No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTGCGTACCCCTACCATCTGG297510.12594429647721725No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAACTG293470.12423405158538854No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTGCGAACCGCTACCATCTGG292530.1238361233184779No Hit
ATACACCATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCATCTGG281700.11925148169013512No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACAGAACCCCTACCAACTGGT275940.11681311273544866No Hit
GATCGCTCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCTACTG268270.11356618740863524No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACTCCGTACCCCTACCATCTGG259940.11003986563909735No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACACCGTACCCCTACCATCTGG259630.10990863397660554No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCTACTG246350.10428683888663397No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACCGAACCCCTACCAAGTGGT246290.10426143921002265No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAGTGGT245380.10387621078141766No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACAGAACCCCTACCAACTGGT236850.10026522342317537No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)