FastQCFastQC Report
Sat 8 Apr 2017
H5GGKBGX2_n01_attga1_-chx_ricetarget_rep2_7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GGKBGX2_n01_attga1_-chx_ricetarget_rep2_7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10585794
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG458100.432749777673739No Hit
CGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGCAGGGAGGAGTCCTG276880.26155808435342687No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA255440.24130452566902397No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC242610.22918450897495266No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG234780.22178780354123648No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG203590.19232378789914106No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC201210.190075491739212No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT166140.156946186559081No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC158230.14947390814519912No Hit
CTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACT153970.14544964695137652No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG152560.14411767317595636No Hit
CCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGC149480.14120811343957762No Hit
TGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCA148600.1403768106577551No Hit
CGGCCCTCGGCGCGCTCGTACTGTTCCACGATGGTGTAGTCCTCGTTGTG148480.14026345118750658No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA148280.14007451873709237No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT145830.13776009621951835No Hit
CGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTTCAAGTAGTCGGGG144300.13631476297384967No Hit
TAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT142670.13477496350297388No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG140650.1328667457537904No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC138160.13051453674613353No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA137530.12991939952732878No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG136140.12860631899695008No Hit
CGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCCAGTACG132720.12537557409486713No Hit
CGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGTTTGAACGA132570.1252338747570565No Hit
GCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGC131070.12381688137894993No Hit
CCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGG122160.11539994071299706No Hit
GTGATGTCCAGCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGG119800.11317053779810943No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT119670.11304773170534019No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG119350.11274543978467746No Hit
GGGACATCCTGTCCCCTCAGTTCCAGTACGGCTCCAAGGCCTACGTGAAG118940.11235812826132835No Hit
GGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCG117350.11085611528053539No Hit
CTTGATCTCGCCCTTCAGGGCGCCGTCCTCGGGGTACATCCGCTCGGTGG115830.10942022865738743No Hit
GTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAA114170.10785208931894953No Hit
AGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGATGAGG110360.10425292613855891No Hit
CGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGAC109050.10301541858834586No Hit
CTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCA107840.10187237726333992No Hit
GGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGAT107030.10110720083916237No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC106260.10037981090506769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTCTA41750.032.59444
CTGACTC38300.031.146672
ACTCTAG41300.030.831675
GCTGACT43050.028.7769071
CGTTTCG17900.027.36081133
TATGCCG17900.027.19324948
CGTATGC19250.026.91935346
TGACTCT47500.026.6606643
TCGTATG18850.026.19035745
AGTCACG21100.024.37762628
ACGTTTC20750.023.94079632
CTCTAGC53150.023.6285446
GTATGCC21550.023.23567247
CTCGTAT19050.022.60650844
TTTCGGA23050.022.0077535
GTTTCGG23200.021.86473534
CTAGCAG55450.021.6384438
CACGTTT23550.021.3929531
TCTAGCA155000.020.8081117
TTCGGAA24600.020.62186236
Produced by FastQC (version 0.11.2)