FastQCFastQC Report
Sat 8 Apr 2017
H5GGKBGX2_n01_attga1_-chx_ricetarget_rep2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GGKBGX2_n01_attga1_-chx_ricetarget_rep2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12242375
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG528850.4319831731996447No Hit
CGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGCAGGGAGGAGTCCTG332740.2717936674869051No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA303030.24752550056667927No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC274560.22427020900764763No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG269760.22034940115786356No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC237760.19421068215930323No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG236760.1933938471905982No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT187740.15335259702467863No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC187130.15285432769376858No Hit
CGGCCCTCGGCGCGCTCGTACTGTTCCACGATGGTGTAGTCCTCGTTGTG181840.14853327070931907No Hit
CTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACT176380.1440733517801897No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG176080.14382830128957821No Hit
CCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGC173810.14197408591061783No Hit
CGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTTCAAGTAGTCGGGG168940.13799609961302445No Hit
TGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCA167360.1367055003624705No Hit
TAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT166340.1358723286943914No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA164460.13433667895322599No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT162290.13256414707113612No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA158040.1290925984541398No Hit
GCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGC155960.1273935817192334No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC155480.127001500934255No Hit
CGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCCAGTACG154010.12580075353025863No Hit
CGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGTTTGAACGA153400.12530248419934858No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG152920.12491040341437018No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG151710.12392203310223711No Hit
GGGACATCCTGTCCCCTCAGTTCCAGTACGGCTCCAAGGCCTACGTGAAG146040.1192905788296797No Hit
GTGATGTCCAGCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGG142830.11666853858013661No Hit
CCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGG141980.11597422885673736No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT141550.1156229898201942No Hit
GGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCG139690.11410367677840288No Hit
GTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAA137870.11261703713535977No Hit
CTTGATCTCGCCCTTCAGGGCGCCGTCCTCGGGGTACATCCGCTCGGTGG137330.11217594625225905No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG131920.10775686907156495No Hit
AGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGATGAGG127700.10430982550362981No Hit
CTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCA127660.10427715210488162No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG125590.10258630371966224No Hit
CGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGAC124640.10181031049939249No Hit
CCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGT124580.10176130040127017No Hit
GGAAGTTGGTGCCGCGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGC123020.10048703785009035No Hit
GGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGAT122520.10007862036573785No Hit
GTCACGGTCACCACGCCGCCGTCCTCGAAGTTCATCACGCGCTCCCACTT122430.10000510521855441No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTCTA46100.028.2299864
CTGACTC40800.027.4386062
ACTCTAG45900.026.3713345
GCTGACT44150.025.761981
TGACTCT52250.022.6308483
TATGCCG17150.022.05132748
TCGTATG18200.020.58378845
TCTAGCA170500.020.1889887
CTCTAGC58050.020.1888356
CGTATGC18400.019.97973646
CTAGCAG56100.019.891698
TAGCAGA63250.018.1408319
CGATTCT130050.018.0771263
TTCTAGC133200.017.623296
ATCGATT129700.017.5118221
ATTCTAG138400.017.1380845
GTATGCC21600.016.85892347
GATTCTA149850.016.8324434
CTCGTAT20500.016.7368844
TCGATTC144400.016.6441562
Produced by FastQC (version 0.11.2)