FastQCFastQC Report
Sat 8 Apr 2017
H5GGKBGX2_n01_attga1_-chx_ricetarget_rep2_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GGKBGX2_n01_attga1_-chx_ricetarget_rep2_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18380394
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG738090.40156375320354937No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC435960.2371875162197285No Hit
CGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGCAGGGAGGAGTCCTG387370.21075173905412475No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG363980.19802622294168448No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG363500.19776507511210045No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA358390.1949849388429867No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC315860.1718461530258818No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT270990.1474342715395546No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC262500.1428152193037864No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT257420.1400514047740217No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG244940.13326156120483598No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA242070.13170011480711458No Hit
TGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCA239730.13042701913789226No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG233970.12729324518288346No Hit
CCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGC231550.12597662487539713No Hit
CTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACT229560.12489394949857983No Hit
TAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT226320.12313120164888738No Hit
CGGCCCTCGGCGCGCTCGTACTGTTCCACGATGGTGTAGTCCTCGTTGTG225580.1227285987449453No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG224250.12200500163380612No Hit
CGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTTCAAGTAGTCGGGG222060.12081351466132881No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG221160.12032386248085868No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC220840.12014976392780263No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA219430.11938264217839945No Hit
GCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGC219390.11936087985926742No Hit
CGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGTTTGAACGA206130.1121466710670076No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT199100.10832194347955762No Hit
CTTGATCTCGCCCTTCAGGGCGCCGTCCTCGGGGTACATCCGCTCGGTGG197020.10719030288469332No Hit
CGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCCAGTACG196930.10714133766664632No Hit
GTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAA196650.10698900143272227No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG195730.10648846809268614No Hit
GGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCG192310.10462778980689967No Hit
GTGATGTCCAGCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGG190920.1038715492170625No Hit
GGGACATCCTGTCCCCTCAGTTCCAGTACGGCTCCAAGGCCTACGTGAAG189320.10300105645178227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACTC66700.032.6237342
GACTCTA75400.032.431744
ACTCTAG74050.030.7553255
GCTGACT77350.028.9095951
TGACTCT85150.026.951763
CTCTAGC96600.023.8656166
CTAGCAG96000.022.9936718
TAGCAGA101150.022.0996389
TATGCCG28700.020.25380548
TCGTATG29700.019.68713645
TCTAGCA260450.019.610057
CGTATGC30100.019.42593646
CTCGTAT29600.018.45165344
GTATGCC32500.017.56171847
TATCTCG32150.017.52672241
CGGGACT22750.016.766661
TCTATCG130500.016.24481216
CTATCGA135350.015.86947917
CGATTCT188600.015.5628173
TTCTAGC193350.015.1984576
Produced by FastQC (version 0.11.2)