FastQCFastQC Report
Sat 8 Apr 2017
H5GGKBGX2_n01_attga1_-chx_ricetarget_rep2_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GGKBGX2_n01_attga1_-chx_ricetarget_rep2_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12396897
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG509860.41128033894288224No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC303060.2444644010513276No Hit
CGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGCAGGGAGGAGTCCTG280840.22654056091617117No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA260020.20974603564101563No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG255520.20611609501958433No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG250520.2020828276624384No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC241620.19490361176671872No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC182180.1469561294249682No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT180950.1459639456551103No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT172870.1394461856059625No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG171290.13817167312110443No Hit
CCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGC170900.13785707826724705No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC168430.13586464419281696No Hit
TGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCA168050.13555811587367386No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA167630.1352193214156736No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG166040.1339367423961012No Hit
CGGCCCTCGGCGCGCTCGTACTGTTCCACGATGGTGTAGTCCTCGTTGTG165500.13350114952152947No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG163770.13210563901595698No Hit
CTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACT161310.1301212714762412No Hit
GCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGC160850.12975021087938376No Hit
TAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT156310.1260880041190953No Hit
CGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTTCAAGTAGTCGGGG155340.12530555025180898No Hit
CGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGTTTGAACGA153880.12412783618352238No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA150890.12171594230394912No Hit
GTGATGTCCAGCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGG147770.11919918347309008No Hit
GGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGAT146510.11818280009908931No Hit
GGGACATCCTGTCCCCTCAGTTCCAGTACGGCTCCAAGGCCTACGTGAAG146420.1181102012866607No Hit
CGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCCAGTACG143800.11599676919151623No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT142810.11519818225480134No Hit
GTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAA141580.11420599848494346No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG137980.11130204598779839No Hit
GGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCG137600.11099551766865531No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG134930.10884175289993939No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC133740.10788183526893866No Hit
CGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGAC132870.10718004674879528No Hit
CTTGATCTCGCCCTTCAGGGCGCCGTCCTCGGGGTACATCCGCTCGGTGG132790.10711551447108096No Hit
CCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGG131230.10585713505565143No Hit
AGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGATGAGG129470.10443742494593608No Hit
GGTACATCCGCTCGGTGGAGGCCTCCCAGCCCATGGTCTTCTTCTGCATT126790.10227559364250585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTCTA46750.031.3544564
CTGACTC43700.028.9002342
GCTGACT45950.028.636381
ACTCTAG48050.028.6131735
TGACTCT54700.025.1989693
CGTATGC21700.023.55486944
TCGTATG21550.023.06787543
TATGCCG21650.022.64048646
CTCTAGC60300.022.3943586
CTCGTAT22700.020.81751342
CTAGCAG62700.020.7548458
GTATGCC25000.019.88635445
TAGCAGA66050.019.1725489
TCTAGCA169250.018.9532957
TCTATCG83350.015.7807027
TTTAATC28550.015.562282
CGATTCT124450.015.3768123
AGATCTA96050.015.2974414
TCTCGTA31650.015.15015141
CTATCGA86150.015.1459898
Produced by FastQC (version 0.11.2)