FastQCFastQC Report
Sat 8 Apr 2017
H5GGKBGX2_n01_attga1_-chx_ricetarget_rep2_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GGKBGX2_n01_attga1_-chx_ricetarget_rep2_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9345855
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG329820.35290511141035247No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC204080.21836418390826737No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG178300.1907797627932383No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG159180.1703214954651019No Hit
CGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGCAGGGAGGAGTCCTG157930.16898400413873316No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA148970.15939686631132197No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC143320.15335140551613521No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT140340.15016282619407215TruSeq Adapter, Index 25 (97% over 44bp)
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT137410.14702774652506378No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT117970.126227081417377No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG117040.12523198787055867No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA115920.12403359564213225No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC114260.12225740716071457No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG110520.11825563311221926No Hit
TGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCA108810.11642594497774683No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG107690.11522755274932042No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG106940.11442505795349917No Hit
CTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACT105260.11262746961085957No Hit
CCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGC104230.11152537675793173No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA103670.11092618064371854No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC102980.11018788543156297No Hit
CGGCCCTCGGCGCGCTCGTACTGTTCCACGATGGTGTAGTCCTCGTTGTG100720.10776970111348828No Hit
GCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGC96130.10285843296306224No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG93520.1000657510736043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC27550.039.2716446
TATGCCG27950.038.0881448
TCGTATG28400.037.84927445
CTCGTAT28500.036.60959644
GTATGCC30600.035.0162947
TATCTCG30300.034.53700641
GACTCTA35800.031.7565544
TCTCGTA34300.031.13143243
CTGACTC34200.030.1740472
ACTCTAG37600.029.3059465
GCTGACT38150.027.2430231
ATGCCGT41150.026.46615649
TGACTCT43000.025.7885063
CCGTCTT42550.025.26818552
CTCTAGC43500.025.0095866
GTCACAC43150.024.81267429
ACTGATA43800.024.59987434
ACGTCTG46600.024.54601315
CAGTCAC44400.024.42999327
AGTCACA44600.024.16353428
Produced by FastQC (version 0.11.2)