FastQCFastQC Report
Sat 8 Apr 2017
H5GGKBGX2_n01_9w-57_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GGKBGX2_n01_9w-57_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11601234
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT26300.026.88535742
TATGCCG28100.025.41866146
TCGTATG27900.025.3472643
CGTATGC29800.023.14515344
TATCTCG29600.023.05234539
AGTCACG33550.021.06728628
ACGTCTG36200.020.00335915
GTCACGC35700.019.79810129
GTATGCC36350.019.45699545
GCCAATA38300.018.35918634
CGCCAAT39700.017.71183633
CACGTCT45400.016.8743714
ATATCTC43850.016.11778338
CCAATAT45900.016.00553735
CAGTCAC45350.015.89437427
CACACGT47400.015.86716812
TCACGCC46300.015.79435630
CGTCTGA46700.015.43104316
ACACGTC49450.015.13863813
ATGCCGT48200.015.11147847
Produced by FastQC (version 0.11.2)