FastQCFastQC Report
Sat 8 Apr 2017
H5GGKBGX2_n01_9w-55_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GGKBGX2_n01_9w-55_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10898573
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAAC177080.16247998705885622No Hit
CAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGC137760.1264018693089453No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGA128720.11810720541120384No Hit
CTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGAT126150.11574909852877069No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA123420.11324418343575805No Hit
GGTAATTCCAGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGC122050.11198713813267112No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG114030.10462837657737394No Hit
CTCCGATCCCGAAGGCCAACACAATAGGACCGGAATCCTATGATGTTATC110320.10122426119456189No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC110210.10112333054978849TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT22750.036.1592742
TCGTATG24400.034.86610843
TATGCCG26150.033.3388546
CGTATGC30750.028.12281844
GTCACAC32600.026.94039329
ACGTCTG35700.024.79057115
GTATGCC35200.024.766945
ATGCCGT37150.024.50616547
AGTCACA38300.023.20517228
CACAGTG39050.022.8444833
CAGTCAC39750.022.27087227
CACGTCT40850.022.17899714
GATTGAC56350.021.4177275
ACACAGT42800.020.35268432
CACACGT44700.019.79918112
CACACAG45850.019.61064131
CTTGTCT67850.019.5998741
ACACGTC46500.019.4841313
CCGTCTT45150.019.46841850
ACAGACT58000.018.99847610
Produced by FastQC (version 0.11.2)