FastQCFastQC Report
Sat 8 Apr 2017
H5GGKBGX2_n01_9w-48_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GGKBGX2_n01_9w-48_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15016364
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC296830.1976710207610844TruSeq Adapter, Index 1 (100% over 50bp)
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA220530.14685978576438344No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA182130.12128768322344875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT41050.054.40408342
TCGTATG44850.053.625243
TATGCCG46650.051.5615646
ACGTCTG51350.047.54974715
GTATGCC53150.045.32078645
CACGTCT54200.045.24305714
ACACGTC54750.045.0439813
GTCACAT54350.044.2931329
ATGCCGT54300.044.10730447
CGTATGC55750.043.14264344
AGTCACA56550.042.69371828
CAGTCAC57150.041.81704727
GTCTGAA63550.038.47656617
CCGTCTT63450.038.02779850
CACGATC57250.037.94997836
GCCGTCT65150.037.1947949
AGCACAC73950.033.34921310
TGCCGTC73900.033.0752348
TCTCGTA70500.031.1765441
TCACGAT84650.030.74830435
Produced by FastQC (version 0.11.2)