FastQCFastQC Report
Sat 8 Apr 2017
H5GGKBGX2_n01_9w-47_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GGKBGX2_n01_9w-47_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10679437
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAAC132350.1239297539748584No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC114120.10685956572429801TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG22600.034.85343643
CTCGTAT21900.034.04314442
TATGCCG22850.034.0148746
CGTATGC26750.029.05487444
TATCTCG25700.029.0019239
AGTCACT30550.025.8857828
ACGTCTG30650.025.56700715
GTATGCC34100.023.40876245
CAGTCAC34550.022.4841827
ATGCCGT35500.021.99455547
ACACGTC37700.021.71385213
CACACGT37450.021.57856212
TCACTAG36600.021.41493230
CACGTCT38750.021.12547714
CACTAGC38450.021.01971231
CTTATCT36150.020.80930337
GCTTATC36900.020.5747436
TTATCTC37700.019.76896738
CCGTCTT40000.019.60990550
GATTGAC46550.019.0139945
Produced by FastQC (version 0.11.2)