FastQCFastQC Report
Sat 8 Apr 2017
H5GGKBGX2_n01_9w-45_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GGKBGX2_n01_9w-45_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10839670
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAAC214410.19780122457602492No Hit
CAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGC159340.14699709493001173No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGA153750.14184011136870403No Hit
CTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGAT153440.14155412480269233No Hit
CTCCGATCCCGAAGGCCAACACAATAGGACCGGAATCCTATGATGTTATC138340.1276238114259936No Hit
GGTAATTCCAGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGC134140.12374915472518996No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA132350.12209781294079985No Hit
CAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAA129420.11939477862333447No Hit
CTCAAACTTCCGTGGCCTAAACGGCCATAGTCCCTCTAAGAAGCTAGCTG127840.11793716967398453No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC115880.10690362344979137TruSeq Adapter, Index 12 (100% over 50bp)
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC110790.10220790854334127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG23650.038.6360443
CTCGTAT22350.038.3715842
TATGCCG24650.037.64109846
CGTATGC31700.029.04756744
ACGTCTG33300.027.94383415
GTATGCC33850.027.72039845
ATGCCGT34500.027.20076847
TGTAATC33850.025.52871336
TAATCTC34000.025.52204738
CAGTCAC36300.025.4477927
CACGTCT36600.025.42430714
CTTGTCT72650.024.4696041
CTTGTAA38550.024.13903234
CCGTCTT38850.023.70632750
AGTCACC39150.023.50524128
TTGTAAT39850.023.26452435
ACACGTC41450.022.70263713
TCTCGTA38650.022.36641741
GATTGAC59550.021.559615
CCAGTCA43900.021.36097126
Produced by FastQC (version 0.11.2)