FastQCFastQC Report
Sat 8 Apr 2017
H5GGKBGX2_n01_9w-41_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GGKBGX2_n01_9w-41_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19509118
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC631870.3238844523878527TruSeq Adapter, Index 4 (100% over 50bp)
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA340580.17457478087938164No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA274510.1407085650924865No Hit
CTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCCG273580.14023186491567685No Hit
GTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATA254150.13027241928620248No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA223970.11480272967747697No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA223500.11456181668489576No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAA213840.10961028581609891No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGA212640.1089951888137639No Hit
CTGGCACAGAGTTAGCCGATGCTTATTCCTCAGATACCGTCATTGTTTCT207340.10627851038678426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT78350.060.8490942
TCGTATG88100.060.042743
TATGCCG88250.059.78856346
ACTGACC92700.057.36266332
ACGTCTG95400.056.58058515
AGTCACT95950.055.6853328
ATGCCGT95300.055.29764647
CACGTCT99050.054.38963314
CAGTCAC100750.053.1716627
ACACGTC102450.052.78948213
CGTATGC100650.052.69811244
GTATGCC101050.052.28337545
GCCGTCT101400.051.8705249
CTGACCA102900.051.74456833
GTCTGAA106700.050.62201717
TGCCGTC110150.048.06786348
CAATCTC99800.047.40146638
CCGTCTT112750.046.96183850
CTGAACT116700.046.19512619
ACCAATC103750.045.99641836