Basic Statistics
Measure | Value |
---|---|
Filename | H5GGKBGX2_n01_9w-41_dr.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 19509118 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC | 63187 | 0.3238844523878527 | TruSeq Adapter, Index 4 (100% over 50bp) |
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA | 34058 | 0.17457478087938164 | No Hit |
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA | 27451 | 0.1407085650924865 | No Hit |
CTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCCG | 27358 | 0.14023186491567685 | No Hit |
GTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATA | 25415 | 0.13027241928620248 | No Hit |
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA | 22397 | 0.11480272967747697 | No Hit |
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA | 22350 | 0.11456181668489576 | No Hit |
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAA | 21384 | 0.10961028581609891 | No Hit |
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGA | 21264 | 0.1089951888137639 | No Hit |
CTGGCACAGAGTTAGCCGATGCTTATTCCTCAGATACCGTCATTGTTTCT | 20734 | 0.10627851038678426 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTCGTAT | 7835 | 0.0 | 60.84909 | 42 |
TCGTATG | 8810 | 0.0 | 60.0427 | 43 |
TATGCCG | 8825 | 0.0 | 59.788563 | 46 |
ACTGACC | 9270 | 0.0 | 57.362663 | 32 |
ACGTCTG | 9540 | 0.0 | 56.580585 | 15 |
AGTCACT | 9595 | 0.0 | 55.68533 | 28 |
ATGCCGT | 9530 | 0.0 | 55.297646 | 47 |
CACGTCT | 9905 | 0.0 | 54.389633 | 14 |
CAGTCAC | 10075 | 0.0 | 53.17166 | 27 |
ACACGTC | 10245 | 0.0 | 52.789482 | 13 |
CGTATGC | 10065 | 0.0 | 52.698112 | 44 |
GTATGCC | 10105 | 0.0 | 52.283375 | 45 |
GCCGTCT | 10140 | 0.0 | 51.87052 | 49 |
CTGACCA | 10290 | 0.0 | 51.744568 | 33 |
GTCTGAA | 10670 | 0.0 | 50.622017 | 17 |
TGCCGTC | 11015 | 0.0 | 48.067863 | 48 |
CAATCTC | 9980 | 0.0 | 47.401466 | 38 |
CCGTCTT | 11275 | 0.0 | 46.961838 | 50 |
CTGAACT | 11670 | 0.0 | 46.195126 | 19 |
ACCAATC | 10375 | 0.0 | 45.996418 | 36 |