FastQCFastQC Report
Sat 8 Apr 2017
H5GGKBGX2_n01_9w-33_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GGKBGX2_n01_9w-33_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16566328
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC2434431.4695048896774228TruSeq Adapter, Index 3 (100% over 50bp)
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA251820.15200713157435974No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA191950.11586755978754011No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC187010.11288560748042656No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAA177850.10735631939679088No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGA169490.10230993856936794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT270150.066.3553542
TATGCCG283100.065.98122446
TCGTATG282950.065.9230143
ACTTAGG286550.065.5976332
CGTATGC290950.064.21091544
GTCACTT293350.064.0397929
AGTCACT293850.064.00342628
GTATGCC292400.063.95409845
ATGCCGT292400.063.89828547
ACGTCTG297200.063.7261415
ACACGTC300550.063.1555113
CACGTCT300600.063.0402614
CAGTCAC301200.062.47737527
GCCGTCT299450.062.31564749
TTAGGCA300700.062.3115334
CCGTCTT303300.061.41307450
GTCTGAA308300.061.4092417
CATCTCG296450.059.77439
TGCCGTC314050.059.55224648
GCTTGAA314300.059.32569559
Produced by FastQC (version 0.11.2)