FastQCFastQC Report
Sat 8 Apr 2017
H5GGKBGX2_n01_9w-07_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GGKBGX2_n01_9w-07_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18897782
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA336700.17816905708828687No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC279570.147937996109808TruSeq Adapter, Index 2 (100% over 50bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA242790.12847539462567617No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA234270.12396692902902574No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGA226190.11969129498900982No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC222520.11774926814162635No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA217460.11507170524032925No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC193880.10259405045523332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT38850.051.08793342
TATGCCG41000.050.64013746
TCGTATG42700.048.12694543
ATGCCGT51150.041.21053347
ACGTCTG53500.039.3632415
CGTATGC53150.038.93028344
GTATGCC54250.038.1425345
TATCTCG52200.038.07649639
CACGTCT57600.036.7435414
CACCGAT57300.036.57483731
CAGTCAC57600.036.20642527
ACACGTC58900.036.17013513
TGTATCT56550.035.4521537
GCCGTCT60700.034.03586649
CGATGTA62600.033.19580534
AGTCACC64750.032.20808828
GTCTGAA66500.031.77371617
TTACCTC39700.031.54573415
TGTTACC40800.031.29542213
CCGATGT68850.030.38539533