Basic Statistics
Measure | Value |
---|---|
Filename | H5GGKBGX2_n01_9w-07_dr.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 18897782 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA | 33670 | 0.17816905708828687 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC | 27957 | 0.147937996109808 | TruSeq Adapter, Index 2 (100% over 50bp) |
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA | 24279 | 0.12847539462567617 | No Hit |
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA | 23427 | 0.12396692902902574 | No Hit |
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGA | 22619 | 0.11969129498900982 | No Hit |
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC | 22252 | 0.11774926814162635 | No Hit |
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA | 21746 | 0.11507170524032925 | No Hit |
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC | 19388 | 0.10259405045523332 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTCGTAT | 3885 | 0.0 | 51.087933 | 42 |
TATGCCG | 4100 | 0.0 | 50.640137 | 46 |
TCGTATG | 4270 | 0.0 | 48.126945 | 43 |
ATGCCGT | 5115 | 0.0 | 41.210533 | 47 |
ACGTCTG | 5350 | 0.0 | 39.36324 | 15 |
CGTATGC | 5315 | 0.0 | 38.930283 | 44 |
GTATGCC | 5425 | 0.0 | 38.14253 | 45 |
TATCTCG | 5220 | 0.0 | 38.076496 | 39 |
CACGTCT | 5760 | 0.0 | 36.74354 | 14 |
CACCGAT | 5730 | 0.0 | 36.574837 | 31 |
CAGTCAC | 5760 | 0.0 | 36.206425 | 27 |
ACACGTC | 5890 | 0.0 | 36.170135 | 13 |
TGTATCT | 5655 | 0.0 | 35.45215 | 37 |
GCCGTCT | 6070 | 0.0 | 34.035866 | 49 |
CGATGTA | 6260 | 0.0 | 33.195805 | 34 |
AGTCACC | 6475 | 0.0 | 32.208088 | 28 |
GTCTGAA | 6650 | 0.0 | 31.773716 | 17 |
TTACCTC | 3970 | 0.0 | 31.545734 | 15 |
TGTTACC | 4080 | 0.0 | 31.295422 | 13 |
CCGATGT | 6885 | 0.0 | 30.385395 | 33 |