FastQCFastQC Report
Sat 8 Apr 2017
H5GGKBGX2_n01_9s-57_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GGKBGX2_n01_9s-57_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18874362
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT591570.3134251637220903TruSeq Adapter, Index 19 (97% over 40bp)
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA200480.10621815985091311No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG193160.10233988306465669No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG82550.055.4348745
CTCGTAT76200.055.3655244
GTCACGT88050.053.92549529
TATGCCG86000.053.83042548
TGAAACG87750.052.82533335
CACGTGA88450.052.7266231
AGTCACG93100.052.1284828
TCACGTG91050.051.8025730
ACGTCTG112200.051.09940315
CGTATGC95500.048.76076546
GAAACGA93650.048.63978636
GTATGCC98150.047.48483347
CACGTCT123200.046.792514
CAGTCAC106550.046.69845227
ACACGTC125400.046.08316413
ATGCCGT103600.044.4148449
GTCTGAA135900.042.497717
AACGATC99200.041.86771438
CCAGTCA130100.040.18203726
CACACGT145450.039.63448712
Produced by FastQC (version 0.11.2)