FastQCFastQC Report
Sat 8 Apr 2017
H5GGKBGX2_n01_9s-55_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GGKBGX2_n01_9s-55_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19908905
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC1764180.8861260827755217TruSeq Adapter, Index 3 (97% over 37bp)
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA199560.10023655243721338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG213750.063.18993843
CTCGTAT207550.062.94379442
TATGCCG218050.061.85354646
GTCACGT220750.061.5784829
CGTATGC220700.061.01290544
AGTCACG228500.059.82831628
ACGTAGA228500.059.4632732
GTATGCC230450.058.57077845
CACGTAG231900.058.56583431
CGTAGAG232600.058.2638433
TCACGTA233950.058.16317730
CAGTCAC237400.057.9691827
ATGCCGT234400.057.57317747
ACGTCTG261150.054.98482515
GCCGTCT253550.053.0621649
TCTCGTA246050.052.7589941
GTAGAGA256350.052.56500634
CCGTCTT256200.052.53028550
CCAGTCA268850.051.85146726
CACGTCT278200.051.7281514
Produced by FastQC (version 0.11.2)