FastQCFastQC Report
Sat 8 Apr 2017
H5GGKBGX2_n01_9s-12_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GGKBGX2_n01_9s-12_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11672313
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC121780.10433236325996398TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT26900.032.1427442
CGTATGC29100.030.19957744
TATGCCG32450.028.0548946
TCGTATG32950.026.88208243
AGTCACG37000.023.83096128
ACGTCTG39950.022.24048815
GTATGCC40200.021.94920545
CACGGCT42000.020.7411731
GCTACAT43950.020.37716935
CACGTCT48700.018.45999514
ATGCCGT49450.018.1989647
CGTCTGA52100.017.32267616
ACACGTC54650.017.1542613
CACACGT53000.016.9623712
TCTCGTA52700.016.73564541
CCGTCTT56150.016.27887750
CCAGTCA56600.016.01148826
CAGTCAC55550.015.93620127
GCCGTCT58150.015.657886549
ACGGCTA56850.015.5682532
Produced by FastQC (version 0.11.2)