FastQCFastQC Report
Sat 8 Apr 2017
H5GGKBGX2_n01_4s-13_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GGKBGX2_n01_4s-13_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11390866
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT25100.032.3538242
TCGTATG27000.030.98960943
TATGCCG28200.029.92169246
CGTATGC31100.027.24294944
ACGTCTG38600.022.92890515
AGTCACG38100.022.59264228
GTATGCC40650.021.01559845
GTCACGA41500.020.57259429
CAGTCAC44650.019.51373927
CACGTCT46600.019.21782114
CACACGT46550.018.78756112
ATGCCGT46850.018.759347
CGTCTGA47950.018.38509416
ACACGTC48500.018.32070213
CACGATC49000.017.9217831
TCACGAT51400.017.0183930
CCAGTCA54100.016.6225826
TCTCGTA50250.016.57658441
CCGTCTT53150.016.208150
TCGGAAG57200.015.2901313
Produced by FastQC (version 0.11.2)