FastQCFastQC Report
Sat 8 Apr 2017
H5GGKBGX2_n01_4s-09_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GGKBGX2_n01_4s-09_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11985211
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG127310.10622257714111166No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAAC120230.10031529691050078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG20000.028.70758843
CTCGTAT19600.028.0389142
TATGCCG23700.024.37648246
CGTATGC25850.022.34782444
CTTGTCT54250.019.0919361
ACGTCTG34350.017.41408215
GATTGAC44600.017.412615
GTATGCC34050.017.27516245
GACTCTA21950.016.7340264
TCTAGTC21450.016.6343527
GGATTGA52700.016.5285384
CCGCATA37350.016.12900264
GACAGAC44000.015.9004849
ACAGACT44600.015.76500910
GTAAGCG18950.015.34046563
CAGTCAC39250.015.33356727
ATGCCGT38650.015.12973847
CACGTCT41250.015.09479714
CGCGTAT8000.014.8712331
TAATTCG57450.014.8042641
Produced by FastQC (version 0.11.2)