FastQCFastQC Report
Sat 8 Apr 2017
H5GGKBGX2_n01_4s-01_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GGKBGX2_n01_4s-01_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15223947
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG32950.023.2680643
CTCGTAT29350.022.66037242
CGTATGC33550.022.5405344
TATGCCG40350.019.17704246
ACGTCTG41000.018.85646615
GTCACAC44800.017.02652229
GATTGAC50650.016.9220985
CACGTCT50600.015.69371614
ACAGACT49300.015.54003310
GTATGCC52700.014.81547945
CTTGTCT73350.014.2660161
CGTCTGA55550.014.16959616
ATGCCGT56600.014.16736447
AGTCACA54900.014.08557328
GGATTGA68200.013.95245554
GACAGAC56750.013.6232629
CAGTCAC59600.013.150969527
CACACGT62950.013.05946612
GGACGTA35700.013.03315454
ACACGTC62200.012.87939713
Produced by FastQC (version 0.11.2)