FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22015472
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4160091.889621081028833No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG47450.017.876364145
CCGTATA39050.013.367215145
CGGTGGG81250.013.295124145
GTAGATA105650.011.940136145
GGGGAAT37200.010.7009451
GGATAGT46000.09.4550861
GTAGGGG35150.09.281468145
GTTAAAA144450.09.1859971
GCGTCGG69100.09.127897145
GCCGTAT184700.08.910222145
CGTATCA170700.08.876511145
CGGGAAT33350.08.8416941
CCGTATC156300.08.4588375140-144
GTAGATG60700.08.360627145
AGTGTAT86800.08.185324145
GTATCAT181800.07.9357634145
AAAGTTC107900.07.9286459
TCGGTGG348200.07.8911443145
CGCCGTA189600.07.8142524140-144
GTTGAAT121850.07.80432751
Produced by FastQC (version 0.11.5)