FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n02_bulk_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n02_bulk_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21944484
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATT581600.2650324336630563No Hit
GTTGAATTAAGATAAATTTTGAAATAAGAATTTTAAATATTTAATCTGTT461570.21033531706646644No Hit
CCTCGATGTTGAATTAAGATAAATTTTGAAATAAGAATTTTAAATATTTA424570.19347458796479333No Hit
GTTTAATAAAAACATTGTTTTTAGAGTATAATTTAAAATTAGTTCTGCTC397960.1813485338730225No Hit
GCATAATTTAATTTTGTAGTATATATATAATTGGGATTATGACCTCGATG369740.16848881021763829No Hit
CAAAAATTAAAGTTCAACTGTTTAATAAAAACATTGTTTTTAGAGTATAA356480.16244628946390355No Hit
GAAACTTTAATAAATATTTTTTCCATAGATGAATGATTATTATATTTTTA346640.15796224691362074No Hit
AGAAACTTTAATAAATATTTTTTCCATAGATGAATGATTATTATATTTTT318690.14522556101114067No Hit
AAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATTATTAT280870.127991161696944No Hit
GGATAGTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAA273680.12471471190664589No Hit
AAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATTATTATATTTT268180.12220838731045122No Hit
TTTTAAATGAAGTCTAGAATGAAGGAGCTAATGAGATTTTAACTTTATAA263730.12018054286443919No Hit
AGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATTATTATA252500.11506308373439084No Hit
AATCAATAGTCTTTTAAATGAAGTCTAGAATGAAGGAGCTAATGAGATTT239800.10927575239408684No Hit
AAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATTATTA230800.10517449396395012No Hit
CTCGATGTTGAATTAAGATAAATTTTGAAATAAGAATTTTAAATATTTAA225350.10269095413681177No Hit
TTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATTA221240.10081804612038268No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGG164550.027.93441145
TCTCGGG153700.027.453472145
GTTAAAA531350.018.9512581
GGATAGT235600.018.770291
TAATCCC115550.018.633919
GGTCATA120250.018.5685144
CCGTATA114750.018.133322145
GTAGATA344250.017.164526145
GAGGTCA127900.015.8708442
GATAGTT279050.015.4577912
GTCATAA150150.015.3538145
AGGTCAT131550.015.3202353
GAGTGTT168800.014.775227145
GGTGGTG406850.013.739413145
TAGTTAA330650.013.5497554
TCGTGTT275850.013.246639145
GGGGAAT133450.013.2009181
TCGCCGG289900.012.929761145
TTAAAAG757450.012.9208872
GTCCTAG50600.012.7513458
Produced by FastQC (version 0.11.5)