FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n02_bulk_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n02_bulk_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23182739
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATT339810.14657888353917112No Hit
CAAAAATTAAAGTTCAACTGTTTAATAAAAACATTGTTTTTAGAGTATAA332600.14346881099769962No Hit
GAAACTTTAATAAATATTTTTTCCATAGATGAATGATTATTATATTTTTA293710.12669339891200948No Hit
GTTTAATAAAAACATTGTTTTTAGAGTATAATTTAAAATTAGTTCTGCTC291490.1257357898909184No Hit
GTTGAATTAAGATAAATTTTGAAATAAGAATTTTAAATATTTAATCTGTT267920.11556874276158653No Hit
AGAAACTTTAATAAATATTTTTTCCATAGATGAATGATTATTATATTTTT244860.10562168689385668No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGG178250.023.916317145
TCTCGGG176950.022.371162145
GTAGATA363850.016.339472145
CCGTATA129750.015.087145
GGTCATA117200.014.3505164
GTTAAAA452500.014.066891
GGATAGT183350.013.166961
GAGTGTT196300.013.074632145
TCGTGTT304900.012.959421145
GGTGGTG421200.012.513898145
CAAAAAT777850.012.2560931
GTAGGGG64800.011.971684145
AAAGTTC488800.011.7762179
GGGGAAT148150.011.695491
TAATCCC137000.011.4300929
GTCATAA151150.010.8875115
GAGGTCA145450.010.8156862
AGGTCAT143650.010.4969933
GTTTAAT569000.010.409551
CGTATCA754250.010.400601145
Produced by FastQC (version 0.11.5)