FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n02_bulk_7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n02_bulk_7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25407332
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATT360270.14179765116620668No Hit
CAAAAATTAAAGTTCAACTGTTTAATAAAAACATTGTTTTTAGAGTATAA304100.11968985960430635No Hit
GTTGAATTAAGATAAATTTTGAAATAAGAATTTTAAATATTTAATCTGTT294320.1158405770428788No Hit
GTTTAATAAAAACATTGTTTTTAGAGTATAATTTAAAATTAGTTCTGCTC291450.11471098185358462No Hit
GAAACTTTAATAAATATTTTTTCCATAGATGAATGATTATTATATTTTTA281660.1108577634204174No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGG200350.024.752068145
TCTCGGG199400.022.32482145
CCGTATA140900.017.700771145
GTAGATA383550.015.632493145
GAGTGTT214350.014.0368395145
GTTAAAA484500.013.4820011
GGATAGT196350.013.3659871
GGGGAAT160550.013.0500131
GTAGGGG75350.012.604708145
GGTGGTG456050.012.447841145
GGTCATA131200.012.3773154
TCGTGTT342700.012.122306145
CAAAAAT822300.011.2497731
TCGCCGG356400.010.639194145
GATAGTT251550.010.5479742
GTTGAAT572900.010.4272971
TAATCCC142950.010.3964989
GCCGTAT892000.010.224935145
CGTATCA820000.010.167841145
GTTTAAT593750.010.0367141
Produced by FastQC (version 0.11.5)