FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n02_bulk_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n02_bulk_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23264545
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATT336190.1445074468466931No Hit
CAAAAATTAAAGTTCAACTGTTTAATAAAAACATTGTTTTTAGAGTATAA322070.13843812548235954No Hit
GTTTAATAAAAACATTGTTTTTAGAGTATAATTTAAAATTAGTTCTGCTC289410.1243995960376616No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGG205600.024.190859145
TCTCGGG198700.023.06054145
GAGTGTT202700.017.311785145
GGATAGT186050.014.1058821
GTTAAAA444050.013.9427141
GTAGGGG78800.013.893158145
GGGGAAT147850.013.7786391
GGTGGTG431150.013.486407145
GTAGATA329250.013.476462145
TCGTGTT334800.013.014854145
CCGTATA128200.012.837725145
GGTCATA116100.011.9889984
CAAAAAT766750.011.7716381
GATAGTT231500.011.2110152
AAAGTTC493400.010.8142469
GTTTAAT563850.010.8131871
GCCGTAT743750.010.333065145
CGTATCA685350.010.293206145
TCGCCGG345150.010.292917145
ATTAAAG610550.010.0334926
Produced by FastQC (version 0.11.5)