FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n02_bulk_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n02_bulk_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23442041
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATT343950.1467235724056621No Hit
GTTTAATAAAAACATTGTTTTTAGAGTATAATTTAAAATTAGTTCTGCTC264570.11286133319193495No Hit
CAAAAATTAAAGTTCAACTGTTTAATAAAAACATTGTTTTTAGAGTATAA260840.11127017481114378No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGG179600.023.292645145
TCTCGGG176650.023.189114145
GTAGATA361950.016.966183145
CCGTATA129000.016.355076145
GGATAGT189150.014.9861191
GAGTGTT191600.014.682012145
GTTAAAA438550.014.0183051
GGTGGTG414550.013.46676145
TCGTGTT317000.012.670677145
GGGGAAT140300.012.6081371
GTAGGGG67050.012.218811145
GATAGTT234150.011.7961762
GGTCATA117100.011.7626784
TAATCCC111300.011.4639049
AGTGTAT263350.010.929656145
TAGTTAA263450.010.7594014
CAAAAAT736050.010.4995011
GCCGTAT807550.010.477334145
CGTATCA741750.010.145866145
TCGCCGG330800.09.994215145
Produced by FastQC (version 0.11.5)