FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n02_bulk_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n02_bulk_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18891807
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATT277290.14677791277456942No Hit
GTTTAATAAAAACATTGTTTTTAGAGTATAATTTAAAATTAGTTCTGCTC228070.12072429069384415No Hit
CAAAAATTAAAGTTCAACTGTTTAATAAAAACATTGTTTTTAGAGTATAA225120.1191627672249669No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGG162550.024.264034145
TCTCGGG161950.022.921345145
CCGTATA99700.016.216637145
GAGTGTT168600.016.168932145
GTAGATA278150.015.639514145
GTAGGGG66650.015.011705145
GGATAGT146650.014.1387081
GTTAAAA372600.013.9703531
GGTGGTG336250.013.950607145
TCGTGTT280500.013.569927145
GGTCATA89900.012.4188084
GGGGAAT110500.011.350361
GTAGGGT75650.011.21316145
TCGCCGG279400.011.132224145
GTAGATG168200.011.121024145
GATAGTT188150.010.865912
CAAAAAT608350.010.8207691
GTTTAAT451000.010.8023571
TAATCCC104350.010.6297929
GCGTCGG178050.010.546512145
Produced by FastQC (version 0.11.5)