FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n02_bulk_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n02_bulk_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24125729
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATT249700.1034994631664809No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGG199100.025.781357145
TCTCGGG199050.021.417015145
CCGTATA147100.017.151825145
GTAGATA375400.016.512606145
GAGTGTT205050.014.178437145
GGTGGTG423000.013.248994145
GTAGGGG70350.011.645499145
GGATAGT158950.011.4937611
GTTAAAA424350.011.4465021
TCGTGTT339650.011.270576145
GGTCATA114150.011.1776584
CAAAAAT708300.011.0849411
GGGGAAT132400.011.0607751
AGTGTAT320350.010.659576145
GCCGTAT913250.010.0663805145
CGTATCA829250.09.897026145
TCGCCGG363600.09.770482145
AAAGTTC455650.09.7054619
ATTAAAG557050.09.6436366
GTAGGGT80900.09.499508145
Produced by FastQC (version 0.11.5)