FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n02_bulk_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n02_bulk_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21071022
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAAATTAAAGTTCAACTGTTTAATAAAAACATTGTTTTTAGAGTATAA257650.12227693559429628No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGG115950.016.695154145
TCTCGGG136050.014.4950695145
GTAGATA246150.013.843564145
GTAGGGG53900.012.913156145
GGATAGT126400.012.1593651
CAAAAAT607350.011.9724771
TCGTGTT273350.011.829481145
GAGTGTT174350.011.352477145
TTAAAGT409050.010.988377
AAAGTTC395050.010.8456229
ATTAAAG472750.010.7345676
TGGTCGC416750.010.629566145
TAAAGTT418750.010.26641758
GGTCATA94850.010.2419674
GCCGTAT273300.010.239945145
ATCTCGG698550.09.444823145
TCGGTGG619000.09.288219145
GGTCGCC413150.09.230557145
GTGGTCG485250.09.084228145
GTGTAGA1044450.09.024136145
Produced by FastQC (version 0.11.5)