FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n02_bulk_24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n02_bulk_24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22380624
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG196350.021.601164145
CCGTATA135200.020.270632145
GTAGATA363500.019.347263145
GTGGGTC99550.018.0602919
CGGTGGG281400.017.983887145
AGGCCCA71350.017.4762738
GGCCCAG73950.016.959869
CGTGGGT107750.016.7531478
ACCAGGC78600.015.4029675
GTCTAAC77450.015.3512771
CTTGGCT145650.014.8329631
GTAGGGG69650.014.052864145
GAGTGTT231050.014.026644145
TCGTGGG136400.013.9783437
CCTCACC91100.013.6877541
TGGCTCG142600.013.5229563
AGTGTAT266250.013.424851145
GGCTCGT146900.012.7323164
CTCGTGG163450.012.4633496
GGGGAAT193900.012.4505491
Produced by FastQC (version 0.11.5)