FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n02_bulk_23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n02_bulk_23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23045057
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG180800.023.739239145
CGGTGGG216900.021.55975145
GTAGATA349300.017.684164145
CCGTATA131800.017.65766145
GAGTGTT202950.012.789026145
AGTGTAT269550.012.668496145
TCGTGTT303850.012.383735145
GGGGAAT178150.012.1267591
GGTGGTG458300.011.595718145
GGATAGT138700.011.2376711
GTAGGGG65400.010.753194145
GTAGGGT76550.010.7022915145
GCCGTAT893150.010.114331145
TCGCCGG359750.010.036263145
CAAAAAT689250.09.6345841
AAGGGGG147750.09.617719145
CGTATCA812450.09.548401145
AGGGGGG185100.09.517949145
GTTAAAA402950.09.3195071
GGTCATA102950.09.2247494
Produced by FastQC (version 0.11.5)