FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n02_bulk_22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n02_bulk_22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22592039
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGG191300.023.762997145
TCTCGGG188700.021.0551145
GTAGATA355950.017.740988145
CCGTATA129000.016.69227145
GAGTGTT190750.014.595377145
GGTGGTG397150.013.691636145
GTAGGGG69850.012.974548145
TCGTGTT317650.012.576256145
AGTGTAT291800.011.156031145
GGGGAAT119750.010.89721
GGATAGT139800.010.7863391
TCGCCGG344050.010.515445145
CGTATCA781500.010.232816145
CAAAAAT630600.09.65702151
GCCGTAT851150.09.599895145
GTTAAAA392100.09.3740871
TAGATCA166700.09.176889145
GTTGAAT392100.09.1152361
GGTCGCC1212300.09.030577145
AAGGGGG149850.08.950837145
Produced by FastQC (version 0.11.5)