FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n02_bulk_21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n02_bulk_21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22831298
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGG196850.023.09279145
TCTCGGG200650.020.234531145
GTAGATA335150.016.721859145
CCGTATA134400.016.021437145
GAGTGTT207650.014.73414145
GGTGGTG409300.013.905038145
TCGTGTT345300.012.61892145
GTAGGGG77400.012.36452145
GGATAGT138400.011.209761
AGTGTAT285600.010.382679145
CAAAAAT619250.010.3023251
GGGGAAT124100.010.10631751
GTTAAAA386900.09.9872781
CGTATCA729250.09.842446145
TCGCCGG355550.09.685839145
GGTCATA102950.09.5769124
TAATCCC106550.09.52561959
GCCGTAT791950.09.484322145
GTAGGGT87950.09.479956145
GGTCGCC1141400.09.337354145
Produced by FastQC (version 0.11.5)