FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n02_bulk_20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n02_bulk_20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22442345
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGG220550.023.373072145
TCTCGGG212550.022.54725145
GAGTGTT212300.017.177948145
GTAGGGG87150.015.806637145
TCGTGTT349850.013.532704145
GGTGGTG420950.013.107123145
CCGTATA120150.012.973822145
GTAGATA298200.012.691593145
GGATAGT147100.012.3208571
GTAGATG210550.012.052194145
GGGGAAT149750.011.2798331
TCGCCGG353750.011.067527145
GTTAAAA397500.010.778641
GCGTCGG222700.010.743529145
GGTCATA103550.010.2914144
CAAAAAT666000.010.0253371
AGTGTAT240150.09.660968145
TAATCCC98650.09.5534419
AGAGCGG305800.09.531075145
CGTATCA649350.09.434766145
Produced by FastQC (version 0.11.5)