FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n02_bulk_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n02_bulk_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21179567
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAAATTAAAGTTCAACTGTTTAATAAAAACATTGTTTTTAGAGTATAA292890.13828894613379017No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG129100.016.342031145
CGGTGGG118650.016.070425145
GTAGGGG59100.014.107489145
GTAGATA235300.012.571225145
TCGTGTT286950.012.077054145
CAAAAAT644450.012.0594781
GAGTGTT163550.012.057511145
GGATAGT141900.012.0062791
GTAGGGT57100.011.427356145
TTAAAGT445550.010.9342657
AAAGTTC432300.010.8333839
ATTAAAG516750.010.7464516
TAAAGTT456850.010.3781738
GGTCATA99950.010.2995254
TAATCCC102450.09.97755059
GTTAAAA377600.09.8109681
GGTCGCC393400.09.564722145
GTGGTCG460600.09.34979145
GTGTAGA984700.09.173888145
GCCGTAT254350.09.149822145
Produced by FastQC (version 0.11.5)