FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n02_bulk_19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n02_bulk_19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23024100
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGG184850.025.023746145
TCTCGGG196200.021.321997145
GTAGATA370550.017.726887145
CCGTATA135000.016.917177145
GAGTGTT200450.015.589146145
GGTGGTG401500.013.254446145
GTAGGGG70000.012.73967145
TCGTGTT326400.012.083697145
AGTGTAT284500.011.544285145
GGGGAAT128550.011.3921041
TCGCCGG347000.011.157397145
GTAGGGT79800.010.539165145
GGATAGT146450.010.4452251
CGTATCA799750.09.836184145
GCCGTAT873400.09.803651145
AAGGGGG145950.09.736505145
GTTAAAA406300.09.4213211
CAAAAAT660300.09.3874911
GGTCGCC1229650.09.0506115145
TGGTCGC1253900.08.973873145
Produced by FastQC (version 0.11.5)