FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n02_bulk_18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n02_bulk_18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22393584
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATT258380.11538126277598082No Hit
CAAAAATTAAAGTTCAACTGTTTAATAAAAACATTGTTTTTAGAGTATAA226050.10094409184344943No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGG199100.022.649721145
TCTCGGG194250.021.087797145
CCGTATA129400.016.080198145
GTAGATA345050.015.002373145
GAGTGTT199950.013.488551145
GGTGGTG398550.013.224991145
GGATAGT154250.013.1600411
GTTAAAA411300.012.6029361
GTAGGGG69000.012.50379145
GGTCATA107450.011.874684
GGGGAAT125100.011.4165081
TCGTGTT302700.011.376926145
TCGCCGG335600.011.10414145
CAAAAAT671900.010.99496651
TAATCCC118300.010.7856069
AAGGGGG169100.010.247031145
AGTGTAT294600.010.188527145
GATAGTT203000.09.8209452
GTTGAAT419750.09.8102931
GCCGTAT919400.09.80979145
Produced by FastQC (version 0.11.5)