FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n02_bulk_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n02_bulk_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23203773
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGG203450.023.305653145
TCTCGGG224600.021.078741145
GTAGATA346500.015.94384145
GAGTGTT222850.015.746134145
GTAGGGG83200.015.249512145
CCGTATA127600.014.488743145
GGTGGTG420600.013.807164145
TCGTGTT360500.013.072219145
GGATAGT146500.011.1838761
TCGCCGG362750.011.05246145
GGGGAAT120650.010.8760921
CGTATCA756700.010.3668145
AGTGTAT277250.010.198452145
GTAGGGT93350.010.174151145
GCCGTAT816950.010.1613455145
GTTAAAA402950.09.9493691
GTAGATG201350.09.7579565145
CAAAAAT653900.09.5125631
GCGTCGG223750.09.299511145
GGTCGCC1166250.09.1631975145
Produced by FastQC (version 0.11.5)