FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n02_bulk_16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n02_bulk_16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22003905
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGG202400.023.175938145
TCTCGGG209700.021.193644145
CCGTATA130850.017.065561145
GAGTGTT208350.015.345769145
GTAGATA340550.015.243184145
GGTGGTG401750.014.65358145
GTTAAAA381300.011.7883131
GGATAGT143800.011.7469151
GTAGGGG77200.011.175661145
GGGGAAT123950.011.0545761
TCGTGTT337500.010.891251145
AGTGTAT288750.010.595807145
CGTATCA754300.010.39023145
CAAAAAT608400.010.2717591
TCGCCGG351550.09.981638145
GCCGTAT830550.09.785508145
GGTCGCC1183350.09.12886145
GTTTAAT413250.09.0173111
GTGGTCG1354100.08.973603145
TTAAAAG480300.08.9507012
Produced by FastQC (version 0.11.5)