FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n02_bulk_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n02_bulk_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23101267
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGG202750.024.67349145
TCTCGGG215350.020.300879145
GAGTGTT216050.015.872643145
GTAGATA332750.015.622246145
GGTGGTG418550.013.822861145
CCGTATA127300.013.49778145
GTAGGGG80550.012.600995145
TCGTGTT340100.012.172277145
GGGGAAT130250.010.4642111
TCGCCGG362100.010.371549145
GTTAAAA396700.010.3255371
GCCGTAT817250.09.971345145
CAAAAAT646600.09.7433991
GGATAGT148200.09.5392181
GTTTAAT459050.09.5390111
GGTCGCC1168650.09.44839145
CGTATCA754650.09.443877145
GGTCATA102100.09.4437564
GTATCAT757600.09.378394145
GTAGATG194150.09.298316145
Produced by FastQC (version 0.11.5)