FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n02_bulk_14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n02_bulk_14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20667265
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATT312350.15113272123815125No Hit
GAAACTTTAATAAATATTTTTTCCATAGATGAATGATTATTATATTTTTA209390.10131480870836079No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGG164450.023.630577145
TCTCGGG150600.023.204142145
CCGTATA109650.017.125156145
GGATAGT161400.016.7990281
GTAGATA312150.016.304863145
GTTAAAA418200.014.8563771
TAATCCC98750.014.0955599
GAGTGTT161450.013.8310795145
GGTCATA107000.013.4151134
GGGGAAT178050.013.3958421
GATAGTT205750.012.8959122
TCGTGTT265550.012.449787145
GGTGGTG383100.011.941547145
TAGTTAA236000.011.4887454
GTAGGGG58850.011.457237145
AGTGTAT235850.011.281674145
GAGGTCA122100.010.7466652
AGGTCAT125800.010.7187513
GTAGGGT62550.010.663602145
GTCATAA138300.010.379065
Produced by FastQC (version 0.11.5)