FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n02_bulk_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n02_bulk_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23107654
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATT310090.13419363125309045No Hit
CAAAAATTAAAGTTCAACTGTTTAATAAAAACATTGTTTTTAGAGTATAA285300.1234655841739711No Hit
GTTTAATAAAAACATTGTTTTTAGAGTATAATTTAAAATTAGTTCTGCTC257340.11136569727069653No Hit
GAAACTTTAATAAATATTTTTTCCATAGATGAATGATTATTATATTTTTA239600.10368858733993506No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGG182950.025.521248145
TCTCGGG174300.023.376926145
CCGTATA127750.015.209761145
GTAGATA358750.015.056111145
GAGTGTT190850.014.131858145
GTAGGGG67100.013.074085145
GGTGGTG408750.012.948324145
GTTAAAA448150.012.8930361
GGGGAAT193200.012.7207551
TCGTGTT291100.012.502877145
GGATAGT176400.012.45272351
TAATCCC106200.012.3557399
GGTCATA114500.012.3464174
CAAAAAT775050.011.7203781
AGTGTAT258500.011.443216145
ACTTAAG141150.010.8885396
AAAGTTC477150.010.48356159
TCGCCGG309850.010.295551145
GCCGTAT840500.010.22184145
GTAGGGT71900.010.083692145
Produced by FastQC (version 0.11.5)