FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n02_bulk_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n02_bulk_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23920478
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATT337210.1409712631996735No Hit
CAAAAATTAAAGTTCAACTGTTTAATAAAAACATTGTTTTTAGAGTATAA307510.12855512335497643No Hit
GAAACTTTAATAAATATTTTTTCCATAGATGAATGATTATTATATTTTTA283680.11859294784995517No Hit
GTTGAATTAAGATAAATTTTGAAATAAGAATTTTAAATATTTAATCTGTT262470.10972606818308564No Hit
GTTTAATAAAAACATTGTTTTTAGAGTATAATTTAAAATTAGTTCTGCTC248240.10377719040564325No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGG193500.023.792511145
TCTCGGG183350.023.527924145
CCGTATA140700.019.220394145
GTAGATA365800.016.569529145
GAGTGTT198400.015.4212055145
TAATCCC116250.014.4057749
GGATAGT186700.013.9403341
GGTCATA122300.013.9301644
GTTAAAA471450.013.5014961
GTAGGGG68950.013.354174145
GGGGAAT148850.012.8094341
GGTGGTG447800.011.5924835145
TCGTGTT316150.011.580992145
TCGCCGG342750.011.507182145
GAGGTCA142300.011.0043112
AGGTCAT146600.010.8793663
CAAAAAT748050.010.8060571
GATAGTT233500.010.6493032
GCCGTAT901100.010.620586145
GTCATAA160400.010.5309235
Produced by FastQC (version 0.11.5)