FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n02_bulk_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n02_bulk_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20174307
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATT438350.21728131727151767No Hit
GTTTAATAAAAACATTGTTTTTAGAGTATAATTTAAAATTAGTTCTGCTC318580.15791372660285186No Hit
CAAAAATTAAAGTTCAACTGTTTAATAAAAACATTGTTTTTAGAGTATAA297640.14753418791535194No Hit
CCTCGATGTTGAATTAAGATAAATTTTGAAATAAGAATTTTAAATATTTA273060.13535037411693993No Hit
GCATAATTTAATTTTGTAGTATATATATAATTGGGATTATGACCTCGATG266160.13193018228581532No Hit
GTTGAATTAAGATAAATTTTGAAATAAGAATTTTAAATATTTAATCTGTT265990.13184591668997603No Hit
GAAACTTTAATAAATATTTTTTCCATAGATGAATGATTATTATATTTTTA236870.11741171580267912No Hit
AGAAACTTTAATAAATATTTTTTCCATAGATGAATGATTATTATATTTTT218030.10807310506378237No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGG274900.024.158348145
TCTCGGG249850.023.214382145
GAGTGTT248900.022.108706145
GTAGGGG146600.018.842455145
GGTCATA107700.018.712674
GGATAGT201900.017.773781
TAATCCC104650.017.6650169
GTTAAAA454850.017.245271
GCGTCGG266650.016.694494145
TCGTGTT396700.016.503347145
GGTGGTG476250.014.979819145
GAGGTCA125350.014.9789742
GATAGTT236700.014.9154852
GTAGATG264100.014.82422145
GTCATAA135900.014.6164195
AGGTCAT125550.014.3776943
CGCCGTT191250.013.040777145
GGGGAAT160500.013.0085351
TCGCCGG327800.012.828194145
TAGTTAA273100.012.6354844
Produced by FastQC (version 0.11.5)