FastQCFastQC Report
Mon 3 Feb 2020
H5GFTBGXF_n02_bulk_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5GFTBGXF_n02_bulk_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24240139
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATT404270.16677709645146838No Hit
CAAAAATTAAAGTTCAACTGTTTAATAAAAACATTGTTTTTAGAGTATAA324280.1337781107608335No Hit
GAAACTTTAATAAATATTTTTTCCATAGATGAATGATTATTATATTTTTA318250.1312905012632147No Hit
GTTGAATTAAGATAAATTTTGAAATAAGAATTTTAAATATTTAATCTGTT310900.12825834043278384No Hit
GTTTAATAAAAACATTGTTTTTAGAGTATAATTTAAAATTAGTTCTGCTC299540.12357189865949202No Hit
CCTCGATGTTGAATTAAGATAAATTTTGAAATAAGAATTTTAAATATTTA282130.11638959661081152No Hit
AGAAACTTTAATAAATATTTTTTCCATAGATGAATGATTATTATATTTTT272430.11238796939241974No Hit
GCATAATTTAATTTTGTAGTATATATATAATTGGGATTATGACCTCGATG260900.10763139600808394No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGG200850.024.25719145
TCTCGGG187800.023.935318145
CCGTATA128800.016.999424145
GAGTGTT202200.016.924036145
GTAGATA366500.016.577274145
TAATCCC121150.015.5585079
GGTCATA126850.015.2592254
GTTAAAA491000.015.0604071
GGATAGT200750.014.66183951
GTAGGGG67800.014.542941145
GGTGGTG450000.013.6784935145
TCGTGTT323500.013.379601145
GAGGTCA148500.012.93692
AGGTCAT149200.012.5846943
GGGGAAT152900.012.090681
GTCATAA164250.011.9171365
CAAAAAT818200.011.5452391
GATAGTT249500.011.4481812
AGTGTAT278400.011.041796145
TCGCCGG346200.011.03638145
Produced by FastQC (version 0.11.5)